Bioinformatics Analysis of Small RNAs in Pima (Gossypium barbadense L.)

Bioinformatics Analysis of Small RNAs in Pima (Gossypium barbadense L.)

Small RNAs (sRNAs) are ~20 to 24 nucleotide single-stranded RNAs that play crucial roles in regulation of gene expression. In plants, sRNAs are classified into microRNAs (miRNAs), repeat-associated siRNAs (ra-siRNAs), phased siRNAs (pha-siRNAs), cis and trans natural antisense transcript siRNAs (cis- and trans-nat siRNAs). Pima (Gossypium barbadense L.) is one of the most economically important...

Nucleic Acid Metabolism during Germination of Pima Cotton (Gossypium barbadense).

The nucleic acid metabolism in long staple Pima cotton (Gossypium barbadense L.) was examined during the first 48 hours of germination at 34 C. Specific activity analysis of pulselabeled nucleic acids indicated that the first significant incorporation of precursors into DNA occurs at approximately 18 hours when the radicles are emerging with a peak of incorporation occurring at 36 hours. Specif...

SNP Marker Discovery in Pima Cotton (Gossypium barbadense L.) Leaf Transcriptomes

The objective of this study was to explore the known narrow genetic diversity and discover single-nucleotide polymorphic (SNP) markers for marker-assisted breeding within Pima cotton (Gossypium barbadense L.) leaf transcriptomes. cDNA from 25-day plants of three diverse cotton genotypes [Pima S6 (PS6), Pima S7 (PS7), and Pima 3-79 (P3-79)] was sequenced on Illumina sequencing platform. A total ...

Gene Flow between Gossypium hirsutum L. and Gossypium barbadense L. is Asymmetric

As both Pima cotton (Gossypium barbadense L.) and Acala (Upland; G. hirsutum L.) are grown in the southwestern United States, understanding gene fl ow within and among these species is important to maintain genetic purity. In small scale and commercial fi elds, gene fl ow decreased exponentially from 1.44% at 1 m to less than 0.50% at 10 m in Acala cotton. Corresponding values for Pima cotton w...

Isolation and Characterization of Genes Differentially Expressed in Fiber of Gossypium barbadense L